human prostate tumor cell lines pc3  (DSMZ)

Journal: Scientific Reports

Article Title: Dissecting the novel molecular interactions of solute carrier family 4 member 4 (SLC4A4) for prostate cancer (PCa) progression

doi: 10.1038/s41598-024-72408-w

Figure Lengend Snippet: Upregulation of SLC4A4 in prostate cancer. ( A , B ) SLC4A4 transcript is highly expressed in several cancers, including prostate adenocarcinoma (PRAD), based on the TCGA study cohort. ( C , D ) A subset of PRAD showed higher SLC4A4 expression in Gleason score 7, 8, and 9 tumors and this was associated with the overall survival of patients. ( E , F ) Immunohistochemical (IHC) analysis of primary prostate tumor samples showed higher SLC4A4 protein expression compared to non-tumor tissues. ( G , H ) Compared to RWPE-1 normal prostate epithelial cells, the SLC4A4 mRNA level was higher in DU145 androgen receptor (AR)-negative prostate cancer (PCa) cells. In AR-positive PCa cells, SLC4A4 mRNA level was elevated in C4-2, followed by VCAP (* P < 0.05). SLC4A4 overexpression was also detected by Western blotting, with PC3 cells having higher SLC4A4 protein level in AR-negative PCa cells. In AR-positive PCa cells, both C4-2 and LNCAP showed higher SLC4A4 protein expression. The results are presented as means ± standard error of three independent experiments.

Article Snippet: Androgen-dependent 22RV1, LNCAP, VCAP, C4-2 and androgen-independent DU145and PC3 human tumor prostate cell lines were purchased from the American Type Culture Collection (ATCC).

Techniques: Expressing, Immunohistochemical staining, Over Expression, Western Blot

Journal: Cells

Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

doi: 10.3390/cells7090129

Figure Lengend Snippet: Adhesion of temsirolimus (TEM)-resistant (PC3 res ) and TEM-sensitive (PC3 par ) prostate cancer cells. ( A ) time-dependent PC3 adhesion to human vein endothelial cells (HUVEC); ( B ) binding to immobilized collagen; ( C ) binding to immobilized fibronectin; ( D ) binding to immobilized laminin. * indicates significant difference to the temsirolimus-free control (PC3 par ). # indicates significant difference between cells exposed to 10 nm of TEM and untreated cells.

Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

Techniques: Binding Assay, Control

Journal: Cells

Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

doi: 10.3390/cells7090129

Figure Lengend Snippet: Motility of temsirolimus (TEM)-resistant (PC3 res ) and TEM-sensitive (PC3 par ) prostate cancer cells. Mean values were calculated from five counts. ( A ) PC3 chemotaxis; ( B ) PC3 migration; ( C ) PC3 invasion. * indicates significant difference to the temsirolimus-free control (PC3 par ). # indicates significant difference between the treated and untreated PC3 sublines with 10 nm TEM.

Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

Techniques: Chemotaxis Assay, Migration, Control

Journal: Cells

Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

doi: 10.3390/cells7090129

Figure Lengend Snippet: Flow activated cell sorting (FACS) analysis of integrin α and β subtype expression on temsirolimus (TEM)-resistant (PC3 res ) and TEM-sensitive (PC3 par ) prostate cancer cells. Mean fluorescence values are shown below the histograms (Par = PC3 parental cells, Res = PC3-resistant cells). One of three independent experiments.

Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

Techniques: FACS, Expressing, Fluorescence

Journal: Cells

Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

doi: 10.3390/cells7090129

Figure Lengend Snippet: ( A ) Integrin protein level in temsirolimus-resistant (PC3 res ) versus temsirolimus-sensitive (PC3 par ) cells quantified by pixel density analysis. Integrins were evaluated three times, integrin-linked kinase (ILK), focal adhesion kinase (FAK), and phosphorylated focal adhesion kinase (pFAK) four times. Representative Western blots are shown on the right panel. ( B ) The integrin gene expression pattern in PC3 res versus PC3 par cells. Values are given as fold difference to PC3 par cells. * indicates a significant difference.

Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

Techniques: Western Blot, Gene Expression

Journal: Cells

Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

doi: 10.3390/cells7090129

Figure Lengend Snippet: Influence of integrin α2, α5, or β1 blockade on PC3 adhesion, chemotaxis, and migration. Values are shown as percentage difference to their respective 100% controls. * indicates significant difference between the PC3 control subline and the PC3 subline treated with the function-blocking antibody. # indicates significant difference between temsirolimus-sensitive (PC3 par ) and temsirolimus-resistant (PC3 res ) cells whose integrin subtype was blocked.

Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

Techniques: Chemotaxis Assay, Migration, Control, Blocking Assay

Journal: Cells

Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

doi: 10.3390/cells7090129

Figure Lengend Snippet: Adhesion of temsirolimus (TEM)-resistant (PC3 res ) versus TEM-sensitive (PC3 par ) prostate cancer cells in the presence of valproic acid (VPA). The figure depicts time-dependent PC3 adhesion to human umbilical vein endothelial cells (HUVEC), binding to immobilized collagen, fibronectin, or laminin. * indicates significant difference to controls not treated with VPA.

Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

Techniques: Binding Assay

Journal: Cells

Article Title: HDAC Inhibition Counteracts Metastatic Re-Activation of Prostate Cancer Cells Induced by Chronic mTOR Suppression

doi: 10.3390/cells7090129

Figure Lengend Snippet: ( A , B ). Chemotactic movement and migration of PC3 res versus PC3 par cells treated with valproic acid (VPA). Values are given as percentage difference to their respective 100% controls. * indicates significant difference to controls not treated with VPA. ( C ). Influence of VPA on integrin α2, α5, or β1 expression. Mean fluorescence units (MFU) are shown as percentage difference to the respective 100% controls (not treated with VPA). ( D ) Influence of VPA on Akt expression. Akt and pAkt levels were quantified by Western blotting and pixel density analysis. Pixel density values of the pAkt/Akt ratio and representative Western blots are shown (Ctrl = control). * indicates significant difference between the PC3 control subline and the PC3 subline treated with VPA. # indicates significant difference between PC3 par and PC3 res cells.

Article Snippet: The human prostate tumor cell line PC3 was obtained from DSMZ (Braunschweig, Germany).

Techniques: Migration, Expressing, Fluorescence, Western Blot, Control